tag:blogger.com,1999:blog-19216099.post4334469974890198127..comments2024-02-16T06:03:12.489-06:00Comments on BrummellBlog: Fixation and PreservationTheBrummellhttp://www.blogger.com/profile/08973380652057861796noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-19216099.post-63094863112427588742007-05-02T08:03:00.000-06:002007-05-02T08:03:00.000-06:00Hmmmm... so, if I'm going to use propylene glycol,...Hmmmm... so, if I'm going to use propylene glycol, I'll need to use air-tight, opaque containers and probably keep everything frozen or at least cool.<BR/><BR/>I'll order some opaque microcentrifuge tubes, then, since the overwhelming majority of my samples will be small enough to fit in that. Thanks for the advice.TheBrummellhttps://www.blogger.com/profile/08973380652057861796noreply@blogger.comtag:blogger.com,1999:blog-19216099.post-91163794372030315102007-05-02T06:57:00.000-06:002007-05-02T06:57:00.000-06:00Good luck on your studies.Just a note of caution t...Good luck on your studies.<BR/>Just a note of caution that propylene glycol will degrade with exposure to air, light, heat to form acids and aldehydes.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-19216099.post-866156460249215612007-05-01T21:06:00.000-06:002007-05-01T21:06:00.000-06:00Unfortunately I don't have any suggestions to offe...Unfortunately I don't have any suggestions to offer you, I'm not a very good chemist. The PG solution sounds like it would be alright if coupled to some form of disinfectant plus hermetic sealing? I'm not sure about the degradation rate of intact nuclei though.<BR/><BR/>My only advice is to figure out what you can and cannot do right away, you don't want to learn that your plans failed utterly 12 months in. Hope I'm not scaring you!Carlohttps://www.blogger.com/profile/00153076425887492166noreply@blogger.com